› 5hzg

X-ray diffraction
3.3Å resolution

The crystal structure of the strigolactone-induced AtD14-D3-ASK1 complex

Released:
DOI: 10.2210/pdb5hzg/pdb
PDB entry 5hzg coloured by chain, front view.
PDB entry 5hzg coloured by chain, side view.
PDB entry 5hzg coloured by chain, top view.
Source organisms:
Primary publication:
DWARF14 is a non-canonical hormone receptor for strigolactone.
Yao R, Ming Z, Yan L, Li S, Wang F, Ma S, Yu C, Yang M, Chen L, Chen L, Li Y, Yan C, Miao D, Sun Z, Yan J, Sun Y, Wang L, Chu J, Fan S, He W, Deng H, Nan F, Li J, Rao Z, Lou Z, Xie D
Nature 536 469-73 (2016)
PMID: 27479325

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-174447 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Strigolactone esterase D14 Chains: A, E
Molecule details ›
Chains: A, E
Length: 267 amino acids
Theoretical weight: 29.65 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q9SQR3 (Residues: 1-267; Coverage: 100%)
Gene names: At3g03990, D14, T11I18.10
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase
F-box/LRR-repeat MAX2 homolog Chains: B, F
Molecule details ›
Chains: B, F
Length: 740 amino acids
Theoretical weight: 81.15 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q5VMP0 (Residues: 1-720; Coverage: 100%)
Gene names: D3, LOC_Os06g06050, OSJNBa0085L11.6-1, Os06g0154200
Sequence domains: F-box-like
SKP1-like protein 1A Chains: C, G
Molecule details ›
Chains: C, G
Length: 169 amino acids
Theoretical weight: 19 KDa
Source organism: Arabidopsis thaliana
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q39255 (Residues: 1-160; Coverage: 100%)
Gene names: ASK1, At1g75950, SKP1, SKP1A, T4O12.17, UIP1
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

1 bound ligand:
Ligand 6OM 2 x 6OM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 106.397Å b: 172.979Å c: 186.845Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.246 0.316
Expression systems:
  • Escherichia coli K-12
  • Insect cell expression vector pTIE1

Quick links

Citations

40 review citations

Strigolactone Signaling and Evolution.
Waters et al. (2017)
39 more

10 mentions without citation

The Many Models of Strigolactone Signaling.
Bürger et al. (2020)
9 more