› 5hzh

X-ray diffraction
2.6Å resolution

Crystal structure of photoinhibitable Rac1 containing C450A mutant LOV2 domain

Released:
DOI: 10.2210/pdb5hzh/pdb
PDB entry 5hzh coloured by chain, front view.
PDB entry 5hzh coloured by chain, side view.
PDB entry 5hzh coloured by chain, top view.
Source organisms:
Primary publication:
Engineering extrinsic disorder to control protein activity in living cells.
Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, Dokholyan NV, Hahn KM
Science 354 1441-1444 (2016)
PMID: 27980211

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related C3 botulinum toxin substrate 1; non-specific serine/threonine protein kinase Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 37.07 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: O49003 (Residues: 386-400, 404-546; Coverage: 17%)
  • Canonical: P63000 (Residues: 48-180; Coverage: 69%)
Gene names: MIG5, NPH1-1, RAC1, TC25
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
3 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 36.05Å b: 74.27Å c: 58.4Å
α: 90° β: 94.62° γ: 90°
R-values:
R R work R free
0.206 0.204 0.255
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Advances in protein structure prediction and design.
Kuhlman et al. (2019)
36 more

1 mention without citation

Engineering extrinsic disorder to control protein activity in living cells.
Dagliyan et al. (2016)