› 5i21

X-ray diffraction
1.55Å resolution

Y55W Hfq from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5i21/pdb
PDB entry 5i21 coloured by chain, front view.
PDB entry 5i21 coloured by chain, side view.
PDB entry 5i21 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PA7
Entry authors: Nikulin AD, Lekontseva NV

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein Hfq Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 82 amino acids
Theoretical weight: 9.14 KDa
Source organism: Pseudomonas aeruginosa PA7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6VD57 (Residues: 1-82; Coverage: 100%)
Gene names: PSPA7_5673, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6
Unit cell:
a: 114.362Å b: 114.362Å c: 28.343Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.188 0.214
Expression system: Escherichia coli BL21(DE3)

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