› 5i49

X-ray diffraction
1.8Å resolution

RNA Editing TUTase 1 from Trypanosoma brucei in complex with UTP analog UMPNPP

Released:
DOI: 10.2210/pdb5i49/pdb
PDB entry 5i49 coloured by chain, front view.
PDB entry 5i49 coloured by chain, side view.
PDB entry 5i49 coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
RNA Editing TUTase 1: structural foundation of substrate recognition, complex interactions and drug targeting.
Rajappa-Titu L, Suematsu T, Munoz-Tello P, Long M, Demir Ö, Cheng KJ, Stagno JR, Luecke H, Amaro RE, Aphasizheva I, Aphasizhev R, Thore S
Nucleic Acids Res 44 10862-10878 (2016)
PMID: 27744351

Function and Biology Details

Reaction catalysed: 
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 512 amino acids
Theoretical weight: 57.65 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WQX5 (Residues: 189-699; Coverage: 52%)
Gene name: KRET1
Sequence domains: Cid1 family poly A polymerase

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand 2KH 1 x 2KH
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21212
Unit cell:
a: 129.38Å b: 58.43Å c: 66.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.207
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes.
Aphasizheva et al. (2020)
1 more

1 mention without citation

RNA Editing TUTase 1: structural foundation of substrate recognition, complex interactions and drug targeting.
Rajappa-Titu et al. (2016)