› 5i5i

X-ray diffraction
2.14Å resolution

Shewanella denitrificans nitrous oxide reductase, app form

Released:
DOI: 10.2210/pdb5i5i/pdb
PDB entry 5i5i coloured by chain, front view.
PDB entry 5i5i coloured by chain, side view.
PDB entry 5i5i coloured by chain, top view.
Source organism: Shewanella denitrificans OS217
Primary publication:
Role of Calcium in Secondary Structure Stabilization during Maturation of Nitrous Oxide Reductase.
Schneider LK, Einsle O
Biochemistry 55 1433-40 (2016)
PMID: 26885878

Function and Biology Details

Reaction catalysed: 
Nitrogen + H(2)O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrous-oxide reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 636 amino acids
Theoretical weight: 70.96 KDa
Source organism: Shewanella denitrificans OS217
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12M27 (Residues: 1-628; Coverage: 100%)
Gene names: Sden_2219, nosZ
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 57.083Å b: 121.624Å c: 178.642Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.237 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 citation in other articles

Coordination chemistry of the CuZ site in nitrous oxide reductase and its synthetic mimics.
Rathnayaka et al. (2021)
7 more