› 5i66

X-ray diffraction
1.62Å resolution

X-ray structure of the Fab fragment of 8B6, a murine monoclonal antibody specific for the human serotonin transporter

Released:
DOI: 10.2210/pdb5i66/pdb
PDB entry 5i66 coloured by chain, front view.
PDB entry 5i66 coloured by chain, side view.
PDB entry 5i66 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
X-ray structures and mechanism of the human serotonin transporter.
Coleman JA, Green EM, Gouaux E
Nature 532 334-9 (2016)
PMID: 27049939

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230482 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
8B6 antibody, heavy chain Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 23.69 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins
8B6 antibody, light chain Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 23.72 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P43212
Unit cell:
a: 81.56Å b: 81.56Å c: 142.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.198
Expression system: Spodoptera frugiperda

Quick links

Citations

66 review citations

Molecular Dynamics Simulation for All.
Hollingsworth et al. (2018)
65 more

4 mentions without citation

X-ray structures and mechanism of the human serotonin transporter.
Coleman et al. (2016)
3 more