› 5i6c

X-ray diffraction
3.7Å resolution

The structure of the eukaryotic purine/H+ symporter, UapA, in complex with Xanthine

Released:
DOI: 10.2210/pdb5i6c/pdb
PDB entry 5i6c coloured by chain, front view.
PDB entry 5i6c coloured by chain, side view.
PDB entry 5i6c coloured by chain, top view.
Source organism: Aspergillus nidulans FGSC A4
Primary publication:
Structure of eukaryotic purine/H(+) symporter UapA suggests a role for homodimerization in transport activity.
Alguel Y, Amillis S, Leung J, Lambrinidis G, Capaldi S, Scull NJ, Craven G, Iwata S, Armstrong A, Mikros E, Diallinas G, Cameron AD, Byrne B
Nat Commun 7 11336 (2016)
PMID: 27088252

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uric acid-xanthine permease Chains: A, B
Molecule details ›
Chains: A, B
Length: 574 amino acids
Theoretical weight: 61.2 KDa
Source organism: Aspergillus nidulans FGSC A4
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q07307 (Residues: 1-574; Coverage: 100%)
Gene names: AN6932, uapA
Sequence domains: Permease family

Ligands and Environments

2 bound ligands:
Ligand XAN 2 x XAN
Ligand LMT 4 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 75.09Å b: 173.83Å c: 82.39Å
α: 90° β: 111.11° γ: 90°
R-values:
R R work R free
0.298 0.296 0.327
Expression system: Saccharomyces cerevisiae

Quick links

Citations

8 review citations

A guide to plasma membrane solute carrier proteins.
Pizzagalli et al. (2021)
7 more

12 mentions without citation

Transporter oligomerization: form and function.
Alguel et al. (2016)
11 more