› 5ifm

X-ray diffraction
2.6Å resolution

Human NONO (p54nrb) Homodimer

Released:
DOI: 10.2210/pdb5ifm/pdb
PDB entry 5ifm coloured by chain, front view.
PDB entry 5ifm coloured by chain, side view.
PDB entry 5ifm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A crystallographic study of human NONO (p54(nrb)): overcoming pathological problems with purification, data collection and noncrystallographic symmetry.
Knott GJ, Panjikar S, Thorn A, Fox AH, Conte MR, Lee M, Bond CS
Acta Crystallogr D Struct Biol 72 761-9 (2016)
PMID: 27303796

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172236 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-POU domain-containing octamer-binding protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 261 amino acids
Theoretical weight: 30.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15233 (Residues: 53-312; Coverage: 55%)
Gene names: NONO, NRB54
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CL 10 x CL
Ligand PRO 2 x PRO
Ligand K 4 x K
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 67.15Å b: 407.177Å c: 68.961Å
α: 90° β: 97.75° γ: 90°
R-values:
R R work R free
0.199 0.197 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

G-quadruplexes offer a conserved structural motif for NONO recruitment to NEAT1 architectural lncRNA.
Simko et al. (2020)
3 more

4 mentions without citation

A crystallographic study of human NONO (p54(nrb)): overcoming pathological problems with purification, data collection and noncrystallographic symmetry.
Knott et al. (2016)
3 more