› 5igp

X-ray diffraction
1.6Å resolution

Macrolide 2'-phosphotransferase type I - complex with GDP and erythromycin

Released:
DOI: 10.2210/pdb5igp/pdb
Model geometry
Fit model/data
PDB entry 5igp coloured by chain, front view.
PDB entry 5igp coloured by chain, side view.
PDB entry 5igp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural Basis for Kinase-Mediated Macrolide Antibiotic Resistance.
Fong DH, Burk DL, Blanchet J, Yan AY, Berghuis AM
Structure 25 750-761.e5 (2017)
PMID: 28416110

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-175238 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 33.27 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47396 (Residues: 1-301; Coverage: 100%)
Gene names: AKG29_00750, AZ95_0038, B6R15_004958, B6R15_005532, BANRA_05393, BK300_27400, BK383_16405, C6669_28275, CR538_26720, CWS33_26285, DS732_30505, EIZ93_26315, ETN48_p0083, FTV93_29750, GRC73_23725, GRC73_24610, HL601_25845, HL601_27150, HMV95_24745, HMV95_26330, IDONEFKE_05306, IH772_26370, J8F57_005313, J8F57_005661, KKS54_p00355, NQD80_25710, NY836_12885, OGM49_25975, PWL68_005175, PWL68_005382, QO046_28115, R8O40_005372, R8O40_005513, RCS105_pI0109, RCS28_PI0046, RCS42_P0046, RCS44_P0046, RCS45_P0047, RCS51_P0074, RCS55TR727_P0021, RCS57_p0100, RCS65_P0021, RCS78_P0037, RCS79_P0002, SAMEA4362930_00091, SAMEA4370330_00104, SAMEA4370365_00007, SAMEA4370473_00005, TUM18780_18910, TUM18780_46330, mph(A), mph2, mphA, orf00017, pCTXM123_C0996_11
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

3 bound ligands:
Ligand GDP 1 x GDP
Ligand ERY 1 x ERY
Ligand IPA 1 x IPA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P3221
Unit cell:
a: 45.14Å b: 45.14Å c: 247.05Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.161 0.182
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

The macrolide antibiotic renaissance.
Dinos GP. (2017)
5 more

2 mentions without citation

The role of adjuvants in overcoming antibacterial resistance due to enzymatic drug modification.
El-Khoury et al. (2022)
1 more