› 5ii8

X-ray diffraction
0.99Å resolution

Orthorhombic crystal structure of red abalone lysin at 0.99 A resolution

Released:
DOI: 10.2210/pdb5ii8/pdb
PDB entry 5ii8 coloured by chain, front view.
PDB entry 5ii8 coloured by chain, side view.
PDB entry 5ii8 coloured by chain, top view.
Source organism: Haliotis rufescens
Primary publication:
Structural Basis of Egg Coat-Sperm Recognition at Fertilization.
Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L
Cell 169 1315-1326.e17 (2017)
PMID: 28622512

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Egg-lysin Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 16.48 KDa
Source organism: Haliotis rufescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04552 (Residues: 19-154; Coverage: 100%)
Sequence domains: Egg lysin (Sperm-lysin)
Structure domains: Fertilization protein

Ligands and Environments

2 bound ligands:
Ligand SO4 13 x SO4
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 45.21Å b: 51.05Å c: 80.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.164 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The hallmarks of cell-cell fusion.
Hernández et al. (2017)
8 more

2 mentions without citation

Structural Basis of Egg Coat-Sperm Recognition at Fertilization.
Raj et al. (2017)
1 more