› 5ii9

X-ray diffraction
2.11Å resolution

Monoclinic crystal structure of red abalone lysin at 2.11 A resolution

Released:
DOI: 10.2210/pdb5ii9/pdb
PDB entry 5ii9 coloured by chain, front view.
PDB entry 5ii9 coloured by chain, side view.
PDB entry 5ii9 coloured by chain, top view.
Source organism: Haliotis rufescens
Primary publication:
Structural Basis of Egg Coat-Sperm Recognition at Fertilization.
Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L
Cell 169 1315-1326.e17 (2017)
PMID: 28622512

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Egg-lysin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 138 amino acids
Theoretical weight: 16.48 KDa
Source organism: Haliotis rufescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04552 (Residues: 19-154; Coverage: 100%)
Sequence domains: Egg lysin (Sperm-lysin)
Structure domains: Fertilization protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 39.19Å b: 69.34Å c: 182.75Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.198 0.196 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The hallmarks of cell-cell fusion.
Hernández et al. (2017)
8 more

2 mentions without citation

Structural Basis of Egg Coat-Sperm Recognition at Fertilization.
Raj et al. (2017)
1 more