› 5iia

X-ray diffraction
1.7Å resolution

Crystal structure of red abalone egg VERL repeat 3 in complex with sperm lysin at 1.7 A resolution (crystal form I)

Released:
DOI: 10.2210/pdb5iia/pdb
PDB entry 5iia coloured by chain, front view.
PDB entry 5iia coloured by chain, side view.
PDB entry 5iia coloured by chain, top view.
Source organism: Haliotis rufescens
Primary publication:
Structural Basis of Egg Coat-Sperm Recognition at Fertilization.
Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L
Cell 169 1315-1326.e17 (2017)
PMID: 28622512

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137912 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Egg-lysin Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 136 amino acids
Theoretical weight: 16.3 KDa
Source organism: Haliotis rufescens
Expression system: Homo sapiens
UniProt:
  • Canonical: P04552 (Residues: 19-154; Coverage: 100%)
Sequence domains: Egg lysin (Sperm-lysin)
Structure domains: Fertilization protein
Vitelline envelope sperm lysin receptor Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 129 amino acids
Theoretical weight: 14.56 KDa
Source organism: Haliotis rufescens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8WR62 (Residues: 340-453; Coverage: 3%)
Gene name: VERL
Sequence domains: Vitelline envelope receptor for lysin

Ligands and Environments

1 bound ligand:
Ligand NAG 8 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 60.49Å b: 60.33Å c: 89.17Å
α: 105.04° β: 89.02° γ: 113.28°
R-values:
R R work R free
0.178 0.177 0.204
Expression system: Homo sapiens

Quick links

Citations

9 review citations

The hallmarks of cell-cell fusion.
Hernández et al. (2017)
8 more

3 mentions without citation

Analysis of molecular diversity, population structure and linkage disequilibrium in a worldwide survey of cultivated barley germplasm (Hordeum vulgare L.).
Malysheva-Otto et al. (2006)
2 more