› 5ik8

X-ray diffraction
2Å resolution

Laminin A2LG45 I-form, G6/7 bound.

Released:
DOI: 10.2210/pdb5ik8/pdb
PDB entry 5ik8 coloured by chain, front view.
PDB entry 5ik8 coloured by chain, side view.
PDB entry 5ik8 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of laminin binding to the LARGE glycans on dystroglycan.
Briggs DC, Yoshida-Moriguchi T, Zheng T, Venzke D, Anderson ME, Strazzulli A, Moracci M, Yu L, Hohenester E, Campbell KP
Nat Chem Biol 12 810-4 (2016)
PMID: 27526028

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178803 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Laminin subunit alpha-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 381 amino acids
Theoretical weight: 41.41 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q60675 (Residues: 2742-3118; Coverage: 12%)
Gene name: Lama2
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BDP, XYS
Carbohydrate polymer
5 bound ligands:
Ligand CA 4 x CA
Ligand NAG 2 x NAG
Ligand FLC 4 x FLC
Ligand EDO 9 x EDO
Ligand 4MU 1 x 4MU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: I212121
Unit cell:
a: 111.54Å b: 126.29Å c: 144Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.21
Expression system: Homo sapiens

Quick links

Citations

19 review citations

Virus recognition of glycan receptors.
Thompson et al. (2019)
18 more

3 mentions without citation

Structural biology of laminins.
Hohenester E. (2019)
2 more