› 5ikn

X-ray diffraction
4.8Å resolution

Crystal Structure of the T7 Replisome in the Absence of DNA

Released:
DOI: 10.2210/pdb5ikn/pdb
PDB entry 5ikn coloured by chain, front view.
PDB entry 5ikn coloured by chain, side view.
PDB entry 5ikn coloured by chain, top view.
Source organisms:
Primary publication:
Hybrid Methods Reveal Multiple Flexibly Linked DNA Polymerases within the Bacteriophage T7 Replisome.
Wallen JR, Zhang H, Weis C, Cui W, Foster BM, Ho CMW, Hammel M, Tainer JA, Gross ML, Ellenberger T
Structure 25 157-166 (2017)
PMID: 28052235

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-132739 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 704 amino acids
Theoretical weight: 79.7 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00581 (Residues: 1-704; Coverage: 100%)
Gene name: 5
Sequence domains: DNA polymerase family A
DNA helicase/primase Chains: D, E, F, G, H, I, J
Molecule details ›
Chains: D, E, F, G, H, I, J
Length: 486 amino acids
Theoretical weight: 53.82 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P03692 (Residues: 64-549; Coverage: 86%)
Gene name: 4
Sequence domains:
Thioredoxin 1 Chains: K, L, M
Molecule details ›
Chains: K, L, M
Length: 105 amino acids
Theoretical weight: 11.41 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA27 (Residues: 4-108; Coverage: 96%)
Gene names: ECs4714, Z5291, trxA
Sequence domains: Thioredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 174.866Å b: 238.091Å c: 243.466Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.277 0.275 0.318
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology.
Brosey et al. (2019)
4 more

4 mentions without citation

TWINKLE and Other Human Mitochondrial DNA Helicases: Structure, Function and Disease.
Peter et al. (2020)
3 more