› 5il9

X-ray diffraction
2.2Å resolution

Crystal structure of Deg9

Released:
DOI: 10.2210/pdb5il9/pdb
PDB entry 5il9 coloured by chain, front view.
PDB entry 5il9 coloured by chain, side view.
PDB entry 5il9 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The crystal structure of Deg9 reveals a novel octameric-type HtrA protease.
Ouyang M, Li X, Zhao S, Pu H, Shen J, Adam Z, Clausen T, Zhang L
Nat Plants 3 973-982 (2017)
PMID: 29180814

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190346 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease Do-like 9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 566 amino acids
Theoretical weight: 62.38 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FL12 (Residues: 65-592; Coverage: 89%)
Gene names: At5g40200, DEGP9, MSN9.10, MSN9.100
Sequence domains:
Structure domains: N-terminal domain of MutM-like DNA repair proteins

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: I4
Unit cell:
a: 132.047Å b: 132.047Å c: 154.157Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.228
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

PDM4, a Pentatricopeptide Repeat Protein, Affects Chloroplast Gene Expression and Chloroplast Development in Arabidopsis thaliana.
Wang et al. (2020)
3 more

1 mention without citation

Functional analysis and cryo-electron microscopy of Campylobacter jejuni serine protease HtrA.
Zarzecka et al. (2020)