› 5ilo

X-ray diffraction
2.71Å resolution

Crystal structure of photoreceptor dehydrogenase from Drosophila melanogaster

Released:
DOI: 10.2210/pdb5ilo/pdb
Model geometry
Fit model/data
PDB entry 5ilo coloured by chain, front view.
PDB entry 5ilo coloured by chain, side view.
PDB entry 5ilo coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural Insights into the Drosophila melanogaster Retinol Dehydrogenase, a Member of the Short-Chain Dehydrogenase/Reductase Family.
Hofmann L, Tsybovsky Y, Alexander NS, Babino D, Leung NY, Montell C, Banerjee S, von Lintig J, Palczewski K
Biochemistry 55 6545-6557 (2016)
PMID: 27809489

Function and Biology Details

Reaction catalysed: 
All-trans-retinol-[cellular-retinol-binding-protein] + NAD(+) = all-trans-retinal-[cellular-retinol-binding-protein] + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fat body protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 29.49 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VV42 (Residues: 19-278; Coverage: 94%)
Gene names: CG4899, Dmel\CG4899, Dmel_CG4899, PDH, Pdh, pcdr, pdh
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 70.159Å b: 62.646Å c: 114.113Å
α: 90° β: 96.26° γ: 90°
R-values:
R R work R free
0.206 0.204 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Carotenoid metabolism at the intestinal barrier.
von Lintig et al. (2020)
5 more

2 mentions without citation

Structural Insights into the Drosophila melanogaster Retinol Dehydrogenase, a Member of the Short-Chain Dehydrogenase/Reductase Family.
Hofmann et al. (2016)
1 more