› 5im8

Solution NMR

Solution Structure of the Microtubule-Targeting COS Domain of MID1

Released:
DOI: 10.2210/pdb5im8/pdb
PDB entry 5im8 coloured by chain, ensemble of 20 models, front view.
PDB entry 5im8 coloured by chain, ensemble of 20 models, side view.
PDB entry 5im8 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the microtubule-targeting COS domain of MID1.
Wright KM, Du H, Dagnachew M, Massiah MA
FEBS J 283 3089-102 (2016)
PMID: 27367845

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Midline-1 Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: O15344 (Residues: 320-379; Coverage: 9%)
Gene names: FXY, MID1, RNF59, TRIM18, XPRF
Sequence domains: TRIM C-terminal subgroup One Signature domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: simulated annealing
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

4 review citations

Targeting TRIM Proteins: A Quest towards Drugging an Emerging Protein Class.
D'Amico et al. (2021)
3 more

1 mention without citation

Solution structure of the microtubule-targeting COS domain of MID1.
Wright et al. (2016)