› 5in3

X-ray diffraction
1.73Å resolution

Crystal structure of glucose-1-phosphate bound nucleotidylated human galactose-1-phosphate uridylyltransferase

Released:
DOI: 10.2210/pdb5in3/pdb
PDB entry 5in3 coloured by chain, front view.
PDB entry 5in3 coloured by chain, side view.
PDB entry 5in3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of classic galactosemia from the structure of human galactose 1-phosphate uridylyltransferase.
McCorvie TJ, Kopec J, Pey AL, Fitzpatrick F, Patel D, Chalk R, Shrestha L, Yue WW
Hum Mol Genet 25 2234-2244 (2016)
PMID: 27005423

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139830 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose-1-phosphate uridylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 45.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07902 (Residues: 1-379; Coverage: 100%)
Gene name: GALT
Sequence domains:
Structure domains: HIT-like

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand H2U 2 x H2U
Ligand EDO 28 x EDO
Ligand G1P 2 x G1P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 59.76Å b: 107.99Å c: 126.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Current and Future Treatments for Classic Galactosemia.
Delnoy et al. (2021)
3 more

8 mentions without citation

Sweet and sour: an update on classic galactosemia.
Coelho et al. (2017)
7 more