› 5iqz

X-ray diffraction
2.33Å resolution

Crystal structure of N-terminal domain of Human SIRT7

Released:
DOI: 10.2210/pdb5iqz/pdb
PDB entry 5iqz coloured by chain, front view.
PDB entry 5iqz coloured by chain, side view.
PDB entry 5iqz coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture.
Priyanka A, Solanki V, Parkesh R, Thakur KG
Proteins 84 1558-63 (2016)
PMID: 27287224

Function and Biology Details

Reaction catalysed: 
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; NAD-dependent protein deacetylase sirtuin-7 Chain: A
Molecule details ›
Chain: A
Length: 443 amino acids
Theoretical weight: 49.16 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: Q9NRC8 (Residues: 2-73; Coverage: 18%)
Gene names: JW3994, SIR2L7, SIRT7, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RRCAT INDUS-2 BEAMLINE BL-21
Spacegroup: P21
Unit cell:
a: 60.49Å b: 49.33Å c: 65.68Å
α: 90° β: 101.2° γ: 90°
R-values:
R R work R free
0.186 0.183 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The Current State of NAD+ -Dependent Histone Deacetylases (Sirtuins) as Novel Therapeutic Targets.
Schiedel et al. (2018)
4 more

6 mentions without citation

Next-generation of selective histone deacetylase inhibitors.
Yang et al. (2019)
5 more