› 5it7

Electron Microscopy
3.6Å resolution

Structure of the Kluyveromyces lactis 80S ribosome in complex with the cricket paralysis virus IRES and eEF2

Released:
DOI: 10.2210/pdb5it7/pdb
PDB entry 5it7 coloured by chain, front view.
PDB entry 5it7 coloured by chain, side view.
PDB entry 5it7 coloured by chain, top view.
Source organisms:
Primary publication:
Structural characterization of ribosome recruitment and translocation by type IV IRES.
Murray J, Savva CG, Shin BS, Dever TE, Ramakrishnan V, Fernández IS
Elife 5 (2016)
PMID: 27159451
Related structures: EMD-8123

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 83-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110131 (preferred)
Entry contents:
78 distinct polypeptide molecules
3 distinct RNA molecules
2 distinct DNA/RNA hybrid molecules
Macromolecules (83 distinct):
Large ribosomal subunit protein uL2 C-terminal domain-containing protein Chain: AA
Molecule details ›
Chain: AA
Length: 249 amino acids
Theoretical weight: 26.84 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CQM3 (Residues: 2-250; Coverage: 98%)
Gene name: KLLA0_D16027g
Sequence domains:
Large ribosomal subunit protein uL3 Chain: BB
Molecule details ›
Chain: BB
Length: 384 amino acids
Theoretical weight: 43.42 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CJR7 (Residues: 2-385; Coverage: 99%)
Gene names: KLLA0F16511g, RPL3
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 C-terminal domain-containing protein Chain: CC
Molecule details ›
Chain: CC
Length: 360 amino acids
Theoretical weight: 38.99 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CW41 (Residues: 3-362; Coverage: 99%)
Gene name: KLLA0_B07139g
Sequence domains:
Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein Chain: DD
Molecule details ›
Chain: DD
Length: 295 amino acids
Theoretical weight: 33.73 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CRR9 (Residues: 3-297; Coverage: 99%)
Gene name: KLLA0_D06941g
Sequence domains:
60S ribosomal protein L6 Chain: EE
Molecule details ›
Chain: EE
Length: 170 amino acids
Theoretical weight: 19.49 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CWE7 (Residues: 6-175; Coverage: 97%)
Gene name: KLLA0_B04686g
Sequence domains: Ribosomal protein L6e
KLLA0D03410p Chain: FF
Molecule details ›
Chain: FF
Length: 222 amino acids
Theoretical weight: 25.11 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CS71 (Residues: 20-241; Coverage: 92%)
Gene name: KLLA0_D03410g
Sequence domains:
60S ribosomal protein L8 Chain: GG
Molecule details ›
Chain: GG
Length: 233 amino acids
Theoretical weight: 25.95 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: B4UN96 (Residues: 23-255; Coverage: 91%)
Gene name: KLLA0_E00573g
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Large ribosomal subunit protein uL6 alpha-beta domain-containing protein Chain: HH
Molecule details ›
Chain: HH
Length: 191 amino acids
Theoretical weight: 21.73 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CLA6 (Residues: 1-191; Coverage: 100%)
Gene name: KLLA0_F04499g
Sequence domains: Ribosomal protein L6
Rpl10 Chain: II
Molecule details ›
Chain: II
Length: 216 amino acids
Theoretical weight: 25 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CRX7 (Residues: 3-218; Coverage: 98%)
Gene name: KLLA0_D05643g
Sequence domains: Ribosomal protein L16p/L10e
KLLA0F08261p Chain: JJ
Molecule details ›
Chain: JJ
Length: 168 amino acids
Theoretical weight: 19.2 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CKT4 (Residues: 6-173; Coverage: 97%)
Gene name: KLLA0_F08261g
Sequence domains:
60S ribosomal protein L13 Chain: LL
Molecule details ›
Chain: LL
Length: 197 amino acids
Theoretical weight: 22.44 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q875M0 (Residues: 2-198; Coverage: 99%)
Sequence domains: Ribosomal protein L13e
KLLA0B13409p Chain: MM
Molecule details ›
Chain: MM
Length: 136 amino acids
Theoretical weight: 14.84 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CVB1 (Residues: 2-137; Coverage: 99%)
Gene name: KLLA0_B13409g
Sequence domains:
Ribosomal protein L15 Chain: NN
Molecule details ›
Chain: NN
Length: 202 amino acids
Theoretical weight: 24.19 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CJL7 (Residues: 2-203; Coverage: 99%)
Gene name: KLLA0_F17633g
Sequence domains: Ribosomal L15
KLLA0F04675p Chain: OO
Molecule details ›
Chain: OO
Length: 198 amino acids
Theoretical weight: 22.25 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CL98 (Residues: 2-199; Coverage: 100%)
Gene name: KLLA0_F04675g
Sequence domains: Ribosomal protein L13
KLLA0A06336p Chain: PP
Molecule details ›
Chain: PP
Length: 180 amino acids
Theoretical weight: 20.32 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CXQ6 (Residues: 3-182; Coverage: 98%)
Gene name: KLLA0_A06336g
Sequence domains: Ribosomal protein L22p/L17e
Large ribosomal subunit protein uL15/eL18 domain-containing protein Chain: QQ
Molecule details ›
Chain: QQ
Length: 184 amino acids
Theoretical weight: 20.48 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CXL8 (Residues: 3-186; Coverage: 99%)
Gene name: KLLA0_A07227g
Sequence domains: Ribosomal protein 60S L18 and 50S L18e
Large ribosomal subunit protein eL19 domain-containing protein Chain: RR
Molecule details ›
Chain: RR
Length: 188 amino acids
Theoretical weight: 21.69 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CNH9 (Residues: 2-189; Coverage: 100%)
Gene name: KLLA0_E12453g
Sequence domains: Ribosomal protein L19e
60S ribosomal protein L20 Chain: SS
Molecule details ›
Chain: SS
Length: 169 amino acids
Theoretical weight: 20.05 KDa
Source organism: Kluyveromyces lactis
KLLA0E23651p Chain: TT
Molecule details ›
Chain: TT
Length: 158 amino acids
Theoretical weight: 17.97 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CM20 (Residues: 2-159; Coverage: 99%)
Gene name: KLLA0_E23651g
Sequence domains: Ribosomal protein L21e
KLLA0D05181p Chain: UU
Molecule details ›
Chain: UU
Length: 100 amino acids
Theoretical weight: 11.42 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CRZ8 (Residues: 9-108; Coverage: 82%)
Gene name: KLLA0_D05181g
Sequence domains: Ribosomal L22e protein family
KLLA0E06997p Chain: VV
Molecule details ›
Chain: VV
Length: 132 amino acids
Theoretical weight: 14 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CP75 (Residues: 5-136; Coverage: 96%)
Gene name: KLLA0_E06997g
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein eL24 Chain: WW
Molecule details ›
Chain: WW
Length: 62 amino acids
Theoretical weight: 7.28 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: P38665 (Residues: 2-63; Coverage: 40%)
Gene names: KLLA0E10857g, RPL24, RPL30
Sequence domains: Ribosomal protein L24e
Large ribosomal subunit protein uL23 Chain: XX
Molecule details ›
Chain: XX
Length: 121 amino acids
Theoretical weight: 13.7 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: P48045 (Residues: 22-142; Coverage: 85%)
Gene names: KLLA0E07458g, RPL25
Sequence domains:
KOW domain-containing protein Chain: YY
Molecule details ›
Chain: YY
Length: 125 amino acids
Theoretical weight: 14.13 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CW97 (Residues: 3-127; Coverage: 98%)
Gene name: KLLA0_B05742g
Sequence domains:
60S ribosomal protein L27 Chain: ZZ
Molecule details ›
Chain: ZZ
Length: 134 amino acids
Theoretical weight: 15.4 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CPN9 (Residues: 3-136; Coverage: 99%)
Gene name: KLLA0_E03455g
Sequence domains: Ribosomal L27e protein family
Large ribosomal subunit protein uL15/eL18 domain-containing protein Chain: aa
Molecule details ›
Chain: aa
Length: 147 amino acids
Theoretical weight: 16.38 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q8J287 (Residues: 2-148; Coverage: 99%)
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
60S ribosomal protein L29 Chain: bb
Molecule details ›
Chain: bb
Length: 57 amino acids
Theoretical weight: 6.5 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CQM1 (Residues: 3-59; Coverage: 92%)
Gene name: KLLA0_D16071g
Sequence domains: Ribosomal L29e protein family
Large ribosomal subunit protein eL30 Chain: cc
Molecule details ›
Chain: cc
Length: 97 amino acids
Theoretical weight: 10.51 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: P38664 (Residues: 9-105; Coverage: 92%)
Gene names: KLLA0E10835g, RPL30, RPL32
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
KLLA0B02937p Chain: dd
Molecule details ›
Chain: dd
Length: 106 amino acids
Theoretical weight: 12.36 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CWM6 (Residues: 5-110; Coverage: 93%)
Gene name: KLLA0_B02937g
Sequence domains: Ribosomal protein L31e
Rpl32 Chain: ee
Molecule details ›
Chain: ee
Length: 122 amino acids
Theoretical weight: 13.94 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CP82 (Residues: 5-126; Coverage: 94%)
Gene name: KLLA0_E06843g
Sequence domains: Ribosomal protein L32
KLLA0D07405p Chain: ff
Molecule details ›
Chain: ff
Length: 105 amino acids
Theoretical weight: 11.86 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CRP7 (Residues: 2-106; Coverage: 98%)
Gene name: KLLA0_D07405g
Sequence domains: Ribosomal protein L35Ae
KLLA0C08371p Chain: gg
Molecule details ›
Chain: gg
Length: 121 amino acids
Theoretical weight: 13.58 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CU17 (Residues: 2-122; Coverage: 97%)
Gene name: KLLA0_C08371g
Sequence domains: Ribosomal protein L34e
KLLA0F05247p Chain: hh
Molecule details ›
Chain: hh
Length: 116 amino acids
Theoretical weight: 13.68 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CL71 (Residues: 3-118; Coverage: 97%)
Gene name: KLLA0_F05247g
Sequence domains: Ribosomal L29 protein
60S ribosomal protein L36 Chain: ii
Molecule details ›
Chain: ii
Length: 98 amino acids
Theoretical weight: 10.93 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CR18 (Residues: 3-100; Coverage: 98%)
Gene name: KLLA0_D12540g
Sequence domains: Ribosomal protein L36e
Ribosomal protein L37 Chain: jj
Molecule details ›
Chain: jj
Length: 85 amino acids
Theoretical weight: 9.68 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CUW0 (Residues: 2-86; Coverage: 97%)
Gene name: KLLA0_C01870g
Sequence domains: Ribosomal protein L37e
Rpl38 Chain: kk
Molecule details ›
Chain: kk
Length: 76 amino acids
Theoretical weight: 8.79 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CSS8 (Residues: 3-78; Coverage: 97%)
Gene name: KLLA0_C18216g
Sequence domains: Ribosomal L38e protein family
Large ribosomal subunit protein eL39 Chain: ll
Molecule details ›
Chain: ll
Length: 49 amino acids
Theoretical weight: 6.11 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P04650 (Residues: 2-50; Coverage: 96%)
Gene names: J0360, RPL39, RPL46, SPB2, YJL189W
Sequence domains: Ribosomal L39 protein
Ubiquitin-like domain-containing protein Chain: mm
Molecule details ›
Chain: mm
Length: 51 amino acids
Theoretical weight: 5.93 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q9Y854 (Residues: 78-128; Coverage: 40%)
Gene name: ubi1
Sequence domains: Ribosomal L40e family
Small ribosomal subunit protein eS32A Chain: nn
Molecule details ›
Chain: nn
Length: 25 amino acids
Theoretical weight: 3.35 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX86 (Residues: 1-25; Coverage: 100%)
Gene names: D1290, RPL41A, RPL47A, YDL184C, YL41A
Sequence domains: Ribosomal protein L41
Large ribosomal subunit protein eL42 Chain: oo
Molecule details ›
Chain: oo
Length: 101 amino acids
Theoretical weight: 11.68 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: P31027 (Residues: 2-102; Coverage: 95%)
Gene names: KLLA0D07832g, RPL41, RPL44
Sequence domains: Ribosomal protein L44
KLLA0E05941p Chain: pp
Molecule details ›
Chain: pp
Length: 87 amino acids
Theoretical weight: 9.5 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CPC5 (Residues: 3-89; Coverage: 95%)
Gene name: KLLA0_E05941g
Sequence domains: Ribosomal L37ae protein family
Ribosomal protein Chain: qq
Molecule details ›
Chain: qq
Length: 217 amino acids
Theoretical weight: 24.55 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CWR9 (Residues: 1-217; Coverage: 100%)
Gene name: KLLA0_B02002g
Sequence domains: Ribosomal protein L1p/L10e family
60S acidic ribosomal protein P0 Chain: rr
Molecule details ›
Chain: rr
Length: 195 amino acids
Theoretical weight: 21.41 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CW89 (Residues: 6-200; Coverage: 63%)
Gene name: KLLA0_B05918g
Sequence domains:
uL11 Chain: KK
Molecule details ›
Chain: KK
Length: 147 amino acids
Theoretical weight: 12.53 KDa
Source organism: Kluyveromyces lactis
Small ribosomal subunit protein uS2 Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 22.88 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CN12 (Residues: 2-207; Coverage: 81%)
Gene names: KLLA0E16171g, RPS0
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein eS1 Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 24.49 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CWD0 (Residues: 20-233; Coverage: 84%)
Gene names: KLLA0B05060g, RPS1
Sequence domains: Ribosomal S3Ae family
S5 DRBM domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 217 amino acids
Theoretical weight: 23.16 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CKL3 (Residues: 39-255; Coverage: 84%)
Gene name: KLLA0_F09812g
Sequence domains:
40S ribosomal protein S3 Chain: D
Molecule details ›
Chain: D
Length: 223 amino acids
Theoretical weight: 24.83 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CRK7 (Residues: 3-225; Coverage: 94%)
Gene name: KLLA0_D08305g
Sequence domains:
40S ribosomal protein S4 Chain: E
Molecule details ›
Chain: E
Length: 260 amino acids
Theoretical weight: 29.49 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CWJ2 (Residues: 2-261; Coverage: 100%)
Gene name: KLLA0_B03652g
Sequence domains:
Small ribosomal subunit protein uS7 domain-containing protein Chain: F
Molecule details ›
Chain: F
Length: 206 amino acids
Theoretical weight: 22.91 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CRA3 (Residues: 22-227; Coverage: 91%)
Gene name: KLLA0_D10659g
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein eS6 Chain: G
Molecule details ›
Chain: G
Length: 226 amino acids
Theoretical weight: 25.81 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CM04 (Residues: 1-226; Coverage: 96%)
Gene names: KLLA0E24090g, RPS6
Sequence domains: Ribosomal protein S6e
40S ribosomal protein S7 Chain: H
Molecule details ›
Chain: H
Length: 184 amino acids
Theoretical weight: 21.03 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CTD6 (Residues: 4-187; Coverage: 97%)
Gene name: KLLA0_C13519g
Sequence domains: Ribosomal protein S7e
40S ribosomal protein S8 Chain: I
Molecule details ›
Chain: I
Length: 200 amino acids
Theoretical weight: 22.51 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CMG3 (Residues: 2-201; Coverage: 100%)
Gene name: KLLA0_E20461g
Sequence domains: Ribosomal protein S8e
KLLA0E23673p Chain: J
Molecule details ›
Chain: J
Length: 182 amino acids
Theoretical weight: 20.88 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CM18 (Residues: 2-183; Coverage: 97%)
Gene name: KLLA0_E23673g
Sequence domains:
Plectin/eS10 N-terminal domain-containing protein Chain: K
Molecule details ›
Chain: K
Length: 96 amino acids
Theoretical weight: 11.42 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CVZ5 (Residues: 1-96; Coverage: 91%)
Gene name: KLLA0_B08173g
Sequence domains: Plectin/S10 domain
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 155 amino acids
Theoretical weight: 17.71 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CX80 (Residues: 2-156; Coverage: 99%)
Gene name: KLLA0_A10483g
Sequence domains:
40S ribosomal protein S12 Chain: M
Molecule details ›
Chain: M
Length: 122 amino acids
Theoretical weight: 13.12 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CLU4 (Residues: 13-134; Coverage: 91%)
Gene name: KLLA0_F00352g
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Small ribosomal subunit protein uS15 N-terminal domain-containing protein Chain: N
Molecule details ›
Chain: N
Length: 150 amino acids
Theoretical weight: 16.86 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CJK0 (Residues: 2-151; Coverage: 99%)
Gene name: KLLA0_F18040g
Sequence domains:
Small ribosomal subunit protein uS11 Chain: O
Molecule details ›
Chain: O
Length: 127 amino acids
Theoretical weight: 13.46 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: P27069 (Residues: 11-137; Coverage: 93%)
Gene names: CRY1, KLLA0B07623g, RPS14
Sequence domains: Ribosomal protein S11
Rps15 Chain: P
Molecule details ›
Chain: P
Length: 123 amino acids
Theoretical weight: 13.97 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CKV4 (Residues: 8-129; Coverage: 86%)
Gene name: KLLA0_F07843g
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein uS9 Chain: Q
Molecule details ›
Chain: Q
Length: 141 amino acids
Theoretical weight: 15.66 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q875N2 (Residues: 3-143; Coverage: 99%)
Gene names: KLLA0E22077g, RPS16
Sequence domains: Ribosomal protein S9/S16
KLLA0B01474p Chain: R
Molecule details ›
Chain: R
Length: 129 amino acids
Theoretical weight: 14.73 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CWU3 (Residues: 2-130; Coverage: 95%)
Gene name: KLLA0_B01474g
Sequence domains: Ribosomal S17
KLLA0B01562p Chain: S
Molecule details ›
Chain: S
Length: 145 amino acids
Theoretical weight: 16.95 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CWT9 (Residues: 2-146; Coverage: 99%)
Gene name: KLLA0_B01562g
Sequence domains: Ribosomal protein S13/S18
KLLA0A07194p Chain: T
Molecule details ›
Chain: T
Length: 143 amino acids
Theoretical weight: 15.75 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CXM0 (Residues: 2-144; Coverage: 99%)
Gene name: KLLA0_A07194g
Sequence domains: Ribosomal protein S19e
Small ribosomal subunit protein uS10 domain-containing protein Chain: U
Molecule details ›
Chain: U
Length: 106 amino acids
Theoretical weight: 12.03 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CIM1 (Residues: 12-117; Coverage: 91%)
Gene name: KLLA0_F25542g
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein eS21 Chain: V
Molecule details ›
Chain: V
Length: 87 amino acids
Theoretical weight: 9.8 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CXT6 (Residues: 1-87; Coverage: 100%)
Gene names: KLLA0A05700g, RPS21
Sequence domains: Ribosomal protein S21e
Small ribosomal subunit protein uS8 Chain: W
Molecule details ›
Chain: W
Length: 129 amino acids
Theoretical weight: 14.51 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CW21 (Residues: 2-130; Coverage: 99%)
Gene names: KLLA0B07601g, RPS22
Sequence domains: Ribosomal protein S8
RPS23 Chain: X
Molecule details ›
Chain: X
Length: 145 amino acids
Theoretical weight: 16.05 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q875M3 (Residues: 1-145; Coverage: 100%)
Sequence domains: Ribosomal protein S12/S23
40S ribosomal protein S24 Chain: Y
Molecule details ›
Chain: Y
Length: 134 amino acids
Theoretical weight: 15.06 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CU44 (Residues: 2-135; Coverage: 99%)
Gene name: KLLA0_C07755g
Sequence domains: Ribosomal protein S24e
40S ribosomal protein S25 Chain: Z
Molecule details ›
Chain: Z
Length: 70 amino acids
Theoretical weight: 7.94 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CW78 (Residues: 36-105; Coverage: 65%)
Gene name: KLLA0_B06182g
Sequence domains: S25 ribosomal protein
40S ribosomal protein S26 Chain: a
Molecule details ›
Chain: a
Length: 100 amino acids
Theoretical weight: 11.45 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CS01 (Residues: 1-100; Coverage: 84%)
Gene name: KLLA0_D05115g
Sequence domains: Ribosomal protein S26e
40S ribosomal protein S27 Chain: b
Molecule details ›
Chain: b
Length: 82 amino acids
Theoretical weight: 8.88 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CNL2 (Residues: 1-82; Coverage: 100%)
Gene name: KLLA0_E11661g
Sequence domains: Ribosomal protein S27
Small ribosomal subunit protein eS28 Chain: c
Molecule details ›
Chain: c
Length: 63 amino acids
Theoretical weight: 7.07 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: P33285 (Residues: 5-67; Coverage: 94%)
Gene names: KLLA0F20383g, RPS28
Sequence domains: Ribosomal protein S28e
Small ribosomal subunit protein uS14 Chain: d
Molecule details ›
Chain: d
Length: 53 amino acids
Theoretical weight: 6.32 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CPG3 (Residues: 4-56; Coverage: 95%)
Gene names: KLLA0E05071g, RPS29
Sequence domains: Ribosomal protein S14p/S29e
40S ribosomal protein S30 Chain: e
Molecule details ›
Chain: e
Length: 55 amino acids
Theoretical weight: 6.33 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CUH5 (Residues: 9-63; Coverage: 87%)
Gene name: KLLA0_C04809g
Sequence domains: Ribosomal protein S30
Small ribosomal subunit protein eS31 Chain: f
Molecule details ›
Chain: f
Length: 69 amino acids
Theoretical weight: 7.96 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: P69061 (Residues: 82-150; Coverage: 46%)
Gene names: KLLA0D18304g, ubi3
Sequence domains: Ribosomal protein S27a
Asc1 Chain: g
Molecule details ›
Chain: g
Length: 324 amino acids
Theoretical weight: 35.61 KDa
Source organism: Kluyveromyces lactis
UniProt:
  • Canonical: Q6CNI7 (Residues: 3-326; Coverage: 99%)
Gene name: KLLA0_E12277g
Sequence domains: WD domain, G-beta repeat
Eft2p Chain: 1
Molecule details ›
Chain: 1
Length: 827 amino acids
Theoretical weight: 91.59 KDa
Source organism: Saccharomyces cerevisiae P283
Expression system: Saccharomyces cerevisiae
5S ribosomal RNA Chain: 7
Molecule details ›
Chain: 7
Length: 121 nucleotides
Theoretical weight: 38.95 KDa
Sequence domains: 5S ribosomal RNA
5.8S ribosomal RNA Chain: 8
Molecule details ›
Chain: 8
Length: 157 nucleotides
Theoretical weight: 50.29 KDa
Sequence domains: 5.8S ribosomal RNA
18S Ribosomal RNA Chain: 2
Molecule details ›
Chain: 2
Length: 1798 nucleotides
Theoretical weight: 579.24 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
25S ribosomal RNA Chain: 5
Molecule details ›
Chain: 5
Length: 3270 nucleotides
Theoretical weight: 1056.71 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
cricket paralysis virus IRES Chain: 4
Molecule details ›
Chain: 4
Length: 190 nucleotides
Theoretical weight: 60.73 KDa
Sequence domains: Cripavirus internal ribosome entry site (IRES)

Ligands and Environments

4 bound ligands:
Ligand MG 80 x MG
Ligand ZN 6 x ZN
Ligand GCP 1 x GCP
Ligand 6EM 1 x 6EM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-8123
Expression system: Saccharomyces cerevisiae

Quick links

Citations

16 review citations

Noncanonical Translation Initiation in Eukaryotes.
Kwan et al. (2019)
15 more

4 mentions without citation

Structural characterization of ribosome recruitment and translocation by type IV IRES.
Murray et al. (2016)
3 more