› 5ith

X-ray diffraction
2.31Å resolution

TIA-1 RRM2 recognition of target oligonucleotide

Released:
DOI: 10.2210/pdb5ith/pdb
PDB entry 5ith coloured by chain, front view.
PDB entry 5ith coloured by chain, side view.
PDB entry 5ith coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
TIA-1 RRM23 binding and recognition of target oligonucleotides.
Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA
Nucleic Acids Res 45 4944-4957 (2017)
PMID: 28184449

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118801 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cytotoxic granule associated RNA binding protein TIA1 Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31483 (Residues: 104-194; Coverage: 24%)
Gene name: TIA1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
DNA (5'-D(*AP*CP*TP*CP*C*TP*TP*TP*TP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 2.96 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P65
Unit cell:
a: 45.43Å b: 45.43Å c: 84.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.228 0.26
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

RNA-binding proteins in tumor progression.
Qin et al. (2020)
4 more

2 mentions without citation

TIA-1 RRM23 binding and recognition of target oligonucleotides.
Waris et al. (2017)
1 more