› 5itz

X-ray diffraction
2.2Å resolution

Crystal structure of the SAC domain of CPAP in a complex with Tubulin and Darpin

Released:
DOI: 10.2210/pdb5itz/pdb
PDB entry 5itz coloured by chain, front view.
PDB entry 5itz coloured by chain, side view.
PDB entry 5itz coloured by chain, top view.
Source organisms:
Primary publication:
Centriolar CPAP/SAS-4 Imparts Slow Processive Microtubule Growth.
Sharma A, Aher A, Dynes NJ, Frey D, Katrukha EA, Jaussi R, Grigoriev I, Croisier M, Kammerer RA, Akhmanova A, Gönczy P, Steinmetz MO
Dev Cell 37 362-376 (2016)
PMID: 27219064

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-160564 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Structure domains:
Centromere protein J Chain: D
Molecule details ›
Chain: D
Length: 129 amino acids
Theoretical weight: 14.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HC77 (Residues: 311-422; Coverage: 8%)
Gene names: CENPJ, CPAP, LAP, LIP1
Designed ankyrin repeat protein (DARPIN) D1 Chain: F
Molecule details ›
Chain: F
Length: 127 amino acids
Theoretical weight: 13.36 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

4 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand GDP 1 x GDP
Ligand LOC 1 x LOC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 61.08Å b: 85.34Å c: 98.69Å
α: 90° β: 91.77° γ: 90°
R-values:
R R work R free
0.179 0.177 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Once and only once: mechanisms of centriole duplication and their deregulation in disease.
Nigg et al. (2018)
12 more

4 mentions without citation

Insight into microtubule disassembly by kinesin-13s from the structure of Kif2C bound to tubulin.
Wang et al. (2017)
3 more