› 5iv9

X-ray diffraction
4.37Å resolution

The LPS Transporter LptDE from Klebsiella pneumoniae, full-length

Released:
DOI: 10.2210/pdb5iv9/pdb
PDB entry 5iv9 coloured by chain, front view.
PDB entry 5iv9 coloured by chain, side view.
PDB entry 5iv9 coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Structural and Functional Characterization of the LPS Transporter LptDE from Gram-Negative Pathogens.
Botos I, Majdalani N, Mayclin SJ, McCarthy JG, Lundquist K, Wojtowicz D, Barnard TJ, Gumbart JC, Buchanan SK
Structure 24 965-976 (2016)
PMID: 27161977

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102210 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
LPS-assembly protein LptD Chain: A
Molecule details ›
Chain: A
Length: 758 amino acids
Theoretical weight: 85.99 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: C4T9I0 (Residues: 25-782; Coverage: 100%)
Gene names: B6I68_11760, BANRA_04367, BL124_00028160, DW281_06475, EAO17_18030, G4V31_07400, GJJ08_022765, GJJ13_021305, GJJ18_04235, GNF00_07920, NCTC11679_04806, NCTC13443_01619, NCTC13465_04526, NCTC8849_00994, NCTC9637_05670, SAMEA3499874_03033, SAMEA3499901_00861, SAMEA3512100_03573, SAMEA3515122_03055, SAMEA3538658_01031, SAMEA3538828_00900, SAMEA3673026_02605, SAMEA3720909_02220, SAMEA4370542_02539, SAMEA4873597_03200, SAMEA4873632_03828, VKR_04247, imp, lptD
Sequence domains:
LPS-assembly lipoprotein LptE Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 19.39 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0J4W1Y0 (Residues: 20-196; Coverage: 90%)
Gene names: CWM98_23195, CWN47_26360, IAP99_18965, NCTC9177_03009, NUKP37_02010, SAMEA3729809_01716, lptE
Sequence domains: Lipopolysaccharide-assembly

Ligands and Environments

1 bound ligand:
Ligand C8E 2 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I222
Unit cell:
a: 131.219Å b: 155.459Å c: 197.757Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.304 0.301 0.344
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Outer Membrane Biogenesis.
Konovalova et al. (2017)
21 more

12 mentions without citation

The lipopolysaccharide transport (Lpt) machinery: A nonconventional transporter for lipopolysaccharide assembly at the outer membrane of Gram-negative bacteria.
Sperandeo et al. (2017)
11 more