› 5iva

X-ray diffraction
2.99Å resolution

The LPS Transporter LptDE from Pseudomonas aeruginosa, core complex

Released:
DOI: 10.2210/pdb5iva/pdb
PDB entry 5iva coloured by chain, front view.
PDB entry 5iva coloured by chain, side view.
PDB entry 5iva coloured by chain, top view.
Source organisms:
Primary publication:
Structural and Functional Characterization of the LPS Transporter LptDE from Gram-Negative Pathogens.
Botos I, Majdalani N, Mayclin SJ, McCarthy JG, Lundquist K, Wojtowicz D, Barnard TJ, Gumbart JC, Buchanan SK
Structure 24 965-976 (2016)
PMID: 27161977

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191092 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
LPS-assembly protein LptD Chain: A
Molecule details ›
Chain: A
Length: 646 amino acids
Theoretical weight: 75.43 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I5U2 (Residues: 300-924; Coverage: 70%)
Gene names: PA0595, imp, lptD, ostA
Sequence domains: LPS transport system D
LPS-assembly lipoprotein LptE Chain: B
Molecule details ›
Chain: B
Length: 192 amino acids
Theoretical weight: 21.32 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HX32 (Residues: 21-207; Coverage: 99%)
Gene names: PA3988, lptE
Sequence domains: Lipopolysaccharide-assembly

Ligands and Environments

1 bound ligand:
Ligand C8E 7 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2221
Unit cell:
a: 148.923Å b: 155.985Å c: 115.654Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.248 0.295
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Lipid transfer proteins: the lipid commute via shuttles, bridges and tubes.
Wong et al. (2019)
28 more

8 mentions without citation

Insertion of proteins and lipopolysaccharide into the bacterial outer membrane.
Botos et al. (2017)
7 more