› 5ivl

X-ray diffraction
2.3Å resolution

CshA Helicase

Released:
DOI: 10.2210/pdb5ivl/pdb
PDB entry 5ivl coloured by chain, front view.
PDB entry 5ivl coloured by chain, side view.
PDB entry 5ivl coloured by chain, top view.
Source organism: Geobacillus stearothermophilus 10
Primary publication:
Structural Insights into a Unique Dimeric DEAD-Box Helicase CshA that Promotes RNA Decay.
Huen J, Lin CL, Golzarroshan B, Yi WL, Yang WZ, Yuan HS
Structure 25 469-481 (2017)
PMID: 28238534

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DEAD-box ATP-dependent RNA helicase CshA Chains: A, B
Molecule details ›
Chains: A, B
Length: 429 amino acids
Theoretical weight: 48.37 KDa
Source organism: Geobacillus stearothermophilus 10
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 58.489Å b: 96.314Å c: 83.773Å
α: 90° β: 95.21° γ: 90°
R-values:
R R work R free
0.205 0.202 0.251
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Roles of the C-Terminal Amino Acids of Non-Hexameric Helicases: Insights from Escherichia coli UvrD.
Yokota H. (2021)
9 other articles

3 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
2 more