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› 5iw7

X-ray diffraction
3.6Å resolution

Crystal structure of yeast Tsr1, a pre-40S ribosome synthesis factor

Released:
DOI: 10.2210/pdb5iw7/pdb
Model geometry
Fit model/data
PDB entry 5iw7 coloured by chain, front view.
PDB entry 5iw7 coloured by chain, side view.
PDB entry 5iw7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Pre-40S ribosome biogenesis factor Tsr1 is an inactive structural mimic of translational GTPases.
McCaughan UM, Jayachandran U, Shchepachev V, Chen ZA, Rappsilber J, Tollervey D, Cook AG
OpenAccess logo Nat Commun 7 11789 (2016)
PMID: 27250689

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome biogenesis protein TSR1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 704 amino acids
Theoretical weight: 81.02 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07381 (Residues: 46-409, 477-788; Coverage: 86%)
Gene names: TSR1, YDL060W
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I03, DIAMOND BEAMLINE I02, DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 65.687Å b: 174.199Å c: 320.474Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.268 0.295
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Ribosome assembly coming into focus.
Klinge et al. (2019)
8 more

5 mentions without citation

Structure of a eukaryotic cytoplasmic pre-40S ribosomal subunit.
Scaiola et al. (2018)
4 more