› 5iw9

X-ray diffraction
2.47Å resolution

Structure of bacteriophage T4 gp25, sheath polymerization initiator

Released:
DOI: 10.2210/pdb5iw9/pdb
PDB entry 5iw9 coloured by chain, front view.
PDB entry 5iw9 coloured by chain, side view.
PDB entry 5iw9 coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Structure of the T4 baseplate and its function in triggering sheath contraction.
Taylor NM, Prokhorov NS, Guerrero-Ferreira RC, Shneider MM, Browning C, Goldie KN, Stahlberg H, Leiman PG
Nature 533 346-52 (2016)
PMID: 27193680

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Baseplate wedge protein gp25 Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 15.12 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P09425 (Residues: 2-132; Coverage: 99%)
Gene name: 25
Sequence domains: Baseplate wedge protein gp25
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P4122
Unit cell:
a: 69.859Å b: 69.859Å c: 222.179Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.217
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Using Force to Punch Holes: Mechanics of Contractile Nanomachines.
Brackmann et al. (2017)
20 more

2 mentions without citation

Molecular assembly and structure of the bacteriophage T4 tail.
Arisaka et al. (2016)
1 more