› 5izu

X-ray diffraction
2.49Å resolution

A new binding site outside the canonical PDZ domain determines the specific interaction between Shank and SAPAP and their function

Released:
DOI: 10.2210/pdb5izu/pdb
PDB entry 5izu coloured by chain, front view.
PDB entry 5izu coloured by chain, side view.
PDB entry 5izu coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A binding site outside the canonical PDZ domain determines the specific interaction between Shank and SAPAP and their function.
Zeng M, Shang Y, Guo T, He Q, Yung WH, Liu K, Zhang M
Proc Natl Acad Sci U S A 113 E3081-90 (2016)
PMID: 27185935

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175440 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SH3 and multiple ankyrin repeat domains protein 3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 139 amino acids
Theoretical weight: 15.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4ACU6 (Residues: 533-665; Coverage: 8%)
Gene names: Kiaa1650, Prosap2, Shank3
Sequence domains: PDZ domain
Disks large-associated protein 3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 15 amino acids
Theoretical weight: 1.72 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q6PFD5 (Residues: 963-977; Coverage: 2%)
Gene names: Dap3, Dlgap3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3112
Unit cell:
a: 72.824Å b: 72.824Å c: 116.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.183 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

International Union of Basic and Clinical Pharmacology. CV. Somatostatin Receptors: Structure, Function, Ligands, and New Nomenclature.
Günther et al. (2018)
4 more

3 mentions without citation

PDZ Domains as Drug Targets.
Christensen et al. (2019)
2 more