› 5j13

X-ray diffraction
2.3Å resolution

Structural basis for TSLP antagonism by the therapeutic antibody Tezepelumab (MEDI9929 / AMG-157)

Released:
DOI: 10.2210/pdb5j13/pdb
PDB entry 5j13 coloured by chain, front view.
PDB entry 5j13 coloured by chain, side view.
PDB entry 5j13 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma.
Verstraete K, Peelman F, Braun H, Lopez J, Van Rompaey D, Dansercoer A, Vandenberghe I, Pauwels K, Tavernier J, Lambrecht BN, Hammad H, De Winter H, Beyaert R, Lippens G, Savvides SN
Nat Commun 8 14937 (2017)
PMID: 28368013

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212677 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thymic stromal lymphopoietin Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q969D9 (Residues: 8-8, 29-159; Coverage: 81%)
Gene name: TSLP
Sequence domains: Thymic stromal lymphopoietin
Structure domains: Thymic stromal lymphopoietin
anti-TSLP Fab-fragment, light chain Chain: B
Molecule details ›
Chain: B
Length: 245 amino acids
Theoretical weight: 25.87 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
anti-TSLP Fab-fragment, heavy chain Chain: C
Molecule details ›
Chain: C
Length: 263 amino acids
Theoretical weight: 28.44 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P3212
Unit cell:
a: 51.726Å b: 51.726Å c: 370.028Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.189 0.215
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens

Quick links

Citations

31 review citations

Targeting cytokines to treat asthma and chronic obstructive pulmonary disease.
Barnes PJ. (2018)
30 more

4 mentions without citation

Modulation of Signaling Mediated by TSLP and IL-7 in Inflammation, Autoimmune Diseases, and Cancer.
Marković et al. (2020)
3 more