5j14

X-ray diffraction
1.92Å resolution

Crystal structure of endoglycoceramidase I from Rhodococ-cus equi in complex with GM3

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-165841 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Putative secreted endoglycosylceramidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 500 amino acids
Theoretical weight: 53.87 KDa
Source organism: Rhodococcus hoagii 103S
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, SIA
Carbohydrate polymer : NEW Components: BGC, GAL
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 192.521Å b: 49.053Å c: 120.135Å
α: 90° β: 114.19° γ: 90°
R-values:
R R work R free
0.167 0.167 0.204
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'