› 5j2u

X-ray diffraction
2.5Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-235088 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Structure domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 143 amino acids
Theoretical weight: 16.84 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
Tubulin--tyrosine ligase Chain: F
Molecule details ›
Chain: F
Length: 384 amino acids
Theoretical weight: 44.38 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P38584 (Residues: 1-373, 374-377; Coverage: 100%)
Gene name: TTL
Sequence domains: Tubulin-tyrosine ligase family
Structure domains:
Monomethyl auristatin F (MMAF) Chains: G, H
Molecule details ›
Chains: G, H
Length: 5 amino acids
Theoretical weight: 732 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

5 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 5 x MG
Ligand CA 1 x CA
Ligand GDP 2 x GDP
Ligand ACP 1 x ACP
1 modified residue:
Ligand MVA 2 x MVA

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 104.567Å b: 155.39Å c: 182.524Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.25
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

25 review citations

Unlocking the potential of antibody-drug conjugates for cancer therapy.
Drago et al. (2021)
24 more

1 mention without citation

Structural Basis of Microtubule Destabilization by Potent Auristatin Anti-Mitotics.
Waight et al. (2016)