› 5j74

X-ray diffraction
2.7Å resolution

Fluorogen activating protein AM2.2 in complex with TO1-2p

Released:
DOI: 10.2210/pdb5j74/pdb
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PDB entry 5j74 coloured by chain, front view.
PDB entry 5j74 coloured by chain, side view.
PDB entry 5j74 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery of Small-Molecule Nonfluorescent Inhibitors of Fluorogen-Fluorogen Activating Protein Binding Pair.
Wu Y, Stauffer SR, Stanfield RL, Tapia PH, Ursu O, Fisher GW, Szent-Gyorgyi C, Evangelisti A, Waller A, Strouse JJ, Carter MB, Bologa C, Gouveia K, Poslusney M, Waggoner AS, Lindsley CW, Jarvik JW, Sklar LA
J Biomol Screen 21 74-87 (2016)
PMID: 26442911

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
scFv AM2.2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 27.26 KDa
Source organism: Homo sapiens
Expression system: Pichia

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand 6GN 2 x 6GN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I222
Unit cell:
a: 79.448Å b: 114.83Å c: 130.995Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.25
Expression system: Pichia

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Citations

1 citations in other articles

A cell surface display fluorescent biosensor for measuring MMP14 activity in real-time.
Braun et al. (2018)

1 mention without citation

A high-affinity human TCR-like antibody detects celiac disease gluten peptide-MHC complexes and inhibits T cell activation.
Frick et al. (2021)

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