› 5j7i

X-ray diffraction
4Å resolution

Crystal structure of a Geobacillus thermoglucosidasius Acetylating Aldehyde Dehydrogenase in complex with ADP

Released:
DOI: 10.2210/pdb5j7i/pdb
PDB entry 5j7i coloured by chain, front view.
PDB entry 5j7i coloured by chain, side view.
PDB entry 5j7i coloured by chain, top view.
Source organism: Parageobacillus thermoglucosidasius C56-YS93
Primary publication:
Structure of an acetylating aldehyde dehydrogenase from the thermophilic ethanologen Geobacillus thermoglucosidasius.
Extance J, Danson MJ, Crennell SJ
Protein Sci 25 2045-2053 (2016)
PMID: 27571338

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165853 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetaldehyde dehydrogenase (Acetylating) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 488 amino acids
Theoretical weight: 52.28 KDa
Source organism: Parageobacillus thermoglucosidasius C56-YS93
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 3 x ADP
Ligand APR 1 x APR
1 modified residue:
Ligand SCY 4 x SCY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 93.054Å b: 109.543Å c: 204.149Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

The quaternary structure of Thermus thermophilus aldehyde dehydrogenase is stabilized by an evolutionary distinct C-terminal arm extension.
Hayes et al. (2018)
3 more