› 5jae

X-ray diffraction
2.5Å resolution

LeuT in the outward-oriented, Na+-free return state, P21 form at pH 6.5

Released:
DOI: 10.2210/pdb5jae/pdb
PDB entry 5jae coloured by chain, front view.
PDB entry 5jae coloured by chain, side view.
PDB entry 5jae coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
A conserved leucine occupies the empty substrate site of LeuT in the Na(+)-free return state.
Malinauskaite L, Said S, Sahin C, Grouleff J, Shahsavar A, Bjerregaard H, Noer P, Severinsen K, Boesen T, Schiøtt B, Sinning S, Nissen P
Nat Commun 7 11673 (2016)
PMID: 27221344

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 58.08 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

1 bound ligand:
Ligand BOG 11 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 81.57Å b: 92.08Å c: 92.47Å
α: 90° β: 95.18° γ: 90°
R-values:
R R work R free
0.223 0.223 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Alternating access mechanisms of LeuT-fold transporters: trailblazing towards the promised energy landscapes.
Kazmier et al. (2017)
7 more

9 mentions without citation

Structural basis for GABAA receptor potentiation by neurosteroids.
Miller et al. (2017)
8 more