› 5jb2

X-ray diffraction
2.2Å resolution

Crystal structure of chicken LGP2 with 5'ppp 10-mer dsRNA and ADP-AlF4-Mg2+ at 2.2 A resolution.

Released:
DOI: 10.2210/pdb5jb2/pdb
PDB entry 5jb2 coloured by chain, front view.
PDB entry 5jb2 coloured by chain, side view.
PDB entry 5jb2 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Analysis of dsRNA Binding to Anti-viral Pattern Recognition Receptors LGP2 and MDA5.
Uchikawa E, Lethier M, Malet H, Brunel J, Gerlier D, Cusack S
Mol Cell 62 586-602 (2016)
PMID: 27203181

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124161 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
RNA helicase Chain: A
Molecule details ›
Chain: A
Length: 680 amino acids
Theoretical weight: 77.46 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: G0YYQ5 (Residues: 2-674; Coverage: 100%)
Sequence domains:
RNA (5'-R(*GPPP*GP*UP*AP*CP*GP*UP*AP*CP*CP*C)-3') Chain: X
Molecule details ›
Chain: X
Length: 11 nucleotides
Theoretical weight: 3.64 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*GPPP*GP*UP*AP*CP*GP*UP*AP*CP*C)-3') Chain: Y
Molecule details ›
Chain: Y
Length: 10 nucleotides
Theoretical weight: 3.34 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand ADP 1 x ADP
Ligand ALF 1 x ALF
Ligand MG 2 x MG
1 modified residue:
Ligand GTP 2 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 70.14Å b: 96.58Å c: 122.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.245
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

38 review citations

RIG-I-like receptors: their regulation and roles in RNA sensing.
Rehwinkel et al. (2020)
37 more

3 mentions without citation

Discrimination of cytosolic self and non-self RNA by RIG-I-like receptors.
Lässig et al. (2017)
2 more