› 5jbg

X-ray diffraction
2Å resolution

Crystal structure of chicken LGP2 with 5'ppp 26-mer hairpin RNA with 3' GG overhang and ADP-AlF4-Mg2+ at 2.0 A resolution.

Released:
DOI: 10.2210/pdb5jbg/pdb
PDB entry 5jbg coloured by chain, front view.
PDB entry 5jbg coloured by chain, side view.
PDB entry 5jbg coloured by chain, top view.
Source organisms:
Primary publication:
Structural Analysis of dsRNA Binding to Anti-viral Pattern Recognition Receptors LGP2 and MDA5.
Uchikawa E, Lethier M, Malet H, Brunel J, Gerlier D, Cusack S
Mol Cell 62 586-602 (2016)
PMID: 27203181

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124162 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA helicase Chain: A
Molecule details ›
Chain: A
Length: 680 amino acids
Theoretical weight: 77.46 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0YYQ5 (Residues: 2-674; Coverage: 100%)
Sequence domains:
RNA (5'-R(*GPPP*GP*AP*GP*CP*GP*UP*GP*CP*CP*GP*GP*GP*CP*AP*CP*GP*CP*UP*CP*CP*GP*G)-3') Chain: X
Molecule details ›
Chain: X
Length: 26 nucleotides
Theoretical weight: 8.57 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

5 bound ligands:
Ligand ZN 1 x ZN
Ligand ADP 1 x ADP
Ligand ALF 1 x ALF
Ligand MG 1 x MG
Ligand SO4 5 x SO4
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 69.86Å b: 97.43Å c: 122.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.218 0.255
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

40 review citations

RIG-I-like receptors: their regulation and roles in RNA sensing.
Rehwinkel et al. (2020)
39 more