› 5jbj

X-ray diffraction
3.58Å resolution

Crystal structure of chicken LGP2 with 5'p 12-mer dsRNA at 3.6 A resolution

Released:
DOI: 10.2210/pdb5jbj/pdb
PDB entry 5jbj coloured by chain, front view.
PDB entry 5jbj coloured by chain, side view.
PDB entry 5jbj coloured by chain, top view.
Source organisms:
Primary publication:
Structural Analysis of dsRNA Binding to Anti-viral Pattern Recognition Receptors LGP2 and MDA5.
Uchikawa E, Lethier M, Malet H, Brunel J, Gerlier D, Cusack S
Mol Cell 62 586-602 (2016)
PMID: 27203181

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124163 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA helicase Chain: A
Molecule details ›
Chain: A
Length: 680 amino acids
Theoretical weight: 77.46 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0YYQ5 (Residues: 2-674; Coverage: 100%)
Sequence domains:
RNA (5'-R(P*GP*GP*UP*AP*GP*CP*GP*CP*UP*AP*CP*C)-3') Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 12 nucleotides
Theoretical weight: 3.83 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P64
Unit cell:
a: 90.09Å b: 90.09Å c: 196.73Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.256 0.253 0.319
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

40 review citations

RIG-I-like receptors: their regulation and roles in RNA sensing.
Rehwinkel et al. (2020)
39 more

2 mentions without citation

Functional Evolution of Avian RIG-I-Like Receptors.
Zheng et al. (2018)
1 more