› 5jch

X-ray diffraction
2.95Å resolution

Crystal structure of chicken MDA5 with 5'p 10-mer dsRNA and ADP-Mg2+ at 2.95 A resolution (untwinned).

Released:
DOI: 10.2210/pdb5jch/pdb
PDB entry 5jch coloured by chain, front view.
PDB entry 5jch coloured by chain, side view.
PDB entry 5jch coloured by chain, top view.
Source organisms:
Primary publication:
Structural Analysis of dsRNA Binding to Anti-viral Pattern Recognition Receptors LGP2 and MDA5.
Uchikawa E, Lethier M, Malet H, Brunel J, Gerlier D, Cusack S
Mol Cell 62 586-602 (2016)
PMID: 27203181

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113007 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA helicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 701 amino acids
Theoretical weight: 81.67 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D9N195 (Residues: 298-994; Coverage: 70%)
Gene name: MDA5
Sequence domains:
RNA (5'-R(P*GP*GP*UP*AP*CP*GP*UP*AP*CP*C)-3') Chains: U, V, X, Y
Molecule details ›
Chains: U, V, X, Y
Length: 10 nucleotides
Theoretical weight: 3.18 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand ADP 2 x ADP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 72.08Å b: 139.73Å c: 103.19Å
α: 90° β: 110.14° γ: 90°
R-values:
R R work R free
0.257 0.256 0.27
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

40 review citations

RIG-I-like receptors: their regulation and roles in RNA sensing.
Rehwinkel et al. (2020)
39 more

1 mention without citation

Functional Evolution of Avian RIG-I-Like Receptors.
Zheng et al. (2018)