› 5jci

X-ray diffraction
1.7Å resolution

Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica

Released:
DOI: 10.2210/pdb5jci/pdb
PDB entry 5jci coloured by chain, front view.
PDB entry 5jci coloured by chain, side view.
PDB entry 5jci coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica.
Park AK, Kim IS, Do H, Jeon BW, Lee CW, Roh SJ, Shin SC, Park H, Kim YS, Kim YH, Yoon HS, Lee JH, Kim HW
Sci Rep 6 33903 (2016)
PMID: 27652777

Function and Biology Details

Reaction catalysed: 
NADH + 2 monodehydroascorbate = NAD(+) + 2 ascorbate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179186 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monodehydroascorbate reductase 3, cytosolic Chain: A
Molecule details ›
Chain: A
Length: 453 amino acids
Theoretical weight: 48.87 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q652L6 (Residues: 4-435; Coverage: 99%)
Gene names: LOC_Os09g39380, MDAR3, MDHAR3, OJ1155_H10.27, Os09g0567300
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P41212
Unit cell:
a: 81.27Å b: 81.27Å c: 120.665Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.143 0.18
Expression system: Escherichia coli

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Citations

4 review citations

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress.
Hasanuzzaman et al. (2019)
3 more

4 mentions without citation

Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica.
Park et al. (2016)
3 more