› 5jcp

X-ray diffraction
2.1Å resolution

RhoGAP domain of ARAP3 in complex with RhoA in the transition state

Released:
DOI: 10.2210/pdb5jcp/pdb
PDB entry 5jcp coloured by chain, front view.
PDB entry 5jcp coloured by chain, side view.
PDB entry 5jcp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for the Specific Recognition of RhoA by the Dual GTPase-activating Protein ARAP3.
Bao H, Li F, Wang C, Wang N, Jiang Y, Tang Y, Wu J, Shi Y
J Biol Chem 291 16709-19 (2016)
PMID: 27311713

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158273 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3; Transforming protein RhoA Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 47.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8WWN8 (Residues: 906-1107; Coverage: 13%)
  • Canonical: P61586 (Residues: 2-181; Coverage: 93%)
Gene names: ARAP3, ARH12, ARHA, CENTD3, RHO12, RHOA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
Ligand ALF 2 x ALF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 56.786Å b: 93.208Å c: 164.283Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.227
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

MicroRNA Regulation of the Small Rho GTPase Regulators-Complexities and Opportunities in Targeting Cancer Metastasis.
Humphries et al. (2020)
3 other articles

5 mentions without citation

Covalent Inhibitors Allosterically Block the Activation of Rho Family Proteins and Suppress Cancer Cell Invasion.
Sun et al. (2020)
4 more