› 5jd0

X-ray diffraction
2.3Å resolution

crystal structure of ARAP3 RhoGAP domain

Released:
DOI: 10.2210/pdb5jd0/pdb
PDB entry 5jd0 coloured by chain, front view.
PDB entry 5jd0 coloured by chain, side view.
PDB entry 5jd0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for the Specific Recognition of RhoA by the Dual GTPase-activating Protein ARAP3.
Bao H, Li F, Wang C, Wang N, Jiang Y, Tang Y, Wu J, Shi Y
J Biol Chem 291 16709-19 (2016)
PMID: 27311713

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186757 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 24.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8WWN8 (Residues: 906-1107; Coverage: 13%)
Gene names: ARAP3, CENTD3
Sequence domains: RhoGAP domain
Structure domains: Rho GTPase activation protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 108.51Å b: 108.51Å c: 95.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.26
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

MicroRNA Regulation of the Small Rho GTPase Regulators-Complexities and Opportunities in Targeting Cancer Metastasis.
Humphries et al. (2020)
6 other articles

2 mentions without citation

Structural Basis for the Specific Recognition of RhoA by the Dual GTPase-activating Protein ARAP3.
Bao et al. (2016)
1 more