› 5jd3

X-ray diffraction
2.3Å resolution

Crystal structure of LAE5, an alpha/beta hydrolase enzyme from the metagenome of Lake Arreo, Spain

Released:
DOI: 10.2210/pdb5jd3/pdb
PDB entry 5jd3 coloured by chain, front view.
PDB entry 5jd3 coloured by chain, side view.
PDB entry 5jd3 coloured by chain, top view.
Source organism: uncultured bacterium
Entry authors: Stogios PJ, Xu X, Nocek B, Cui H, Yim V, Martinez-Martinez M, Alcaide M, Ferrer M, Savchenko A

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-165859 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LAE5 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 238 amino acids
Theoretical weight: 26.79 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
Structure domains: SGNH hydrolase

Ligands and Environments

3 bound ligands:
Ligand CL 4 x CL
Ligand PEG 2 x PEG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 66.591Å b: 129.452Å c: 129.848Å
α: 90° β: 97.49° γ: 90°
R-values:
R R work R free
0.186 0.184 0.236
Expression system: Escherichia coli BL21(DE3)

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