› 5jd4

X-ray diffraction
2.05Å resolution

Crystal structure of LAE6 Ser161Ala mutant, an alpha/beta hydrolase enzyme from the metagenome of Lake Arreo, Spain

Released:
DOI: 10.2210/pdb5jd4/pdb
PDB entry 5jd4 coloured by chain, front view.
PDB entry 5jd4 coloured by chain, side view.
PDB entry 5jd4 coloured by chain, top view.
Source organism: uncultured bacterium
Entry authors: Stogios PJ, Xu X, Alcaide M, Yim V, Cui H, Martinez-Martinez M, Ferrer M, Savchenko A

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165861 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LAE6 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 336 amino acids
Theoretical weight: 36.39 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
Structure domains: alpha/beta hydrolase

Ligands and Environments

5 bound ligands:
Ligand SO4 10 x SO4
Ligand GOL 27 x GOL
Ligand CL 8 x CL
Ligand PE3 3 x PE3
Ligand BEN 8 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 90.275Å b: 90.098Å c: 110.76Å
α: 68.02° β: 79.6° γ: 67.57°
R-values:
R R work R free
0.169 0.169 0.211
Expression system: Escherichia coli BL21(DE3)

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