› 5jd7

X-ray diffraction
1.75Å resolution

PKG I's Carboxyl Terminal Cyclic Nucleotide Binding Domain (CNB-B) in a complex with PET-cGMP

Released:
DOI: 10.2210/pdb5jd7/pdb
PDB entry 5jd7 coloured by chain, front view.
PDB entry 5jd7 coloured by chain, side view.
PDB entry 5jd7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis of Analog Specificity in PKG I and II.
Campbell JC, Henning P, Franz E, Sankaran B, Herberg FW, Kim C
ACS Chem Biol 12 2388-2398 (2017)
PMID: 28793191

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 14.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13976 (Residues: 204-336; Coverage: 20%)
Gene names: PRKG1, PRKG1B, PRKGR1A, PRKGR1B
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand 6JR 1 x 6JR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41212
Unit cell:
a: 46.29Å b: 46.29Å c: 102.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.193 0.226
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

New cGMP analogues restrain proliferation and migration of melanoma cells.
Vighi et al. (2018)
5 more

2 mentions without citation

Cyclic nucleotide selectivity of protein kinase G isozymes.
Kim et al. (2021)
1 more