› 5jdq

X-ray diffraction
2.5Å resolution

Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 100 mM Na+ and 10mM Sr2+

Released:
DOI: 10.2210/pdb5jdq/pdb
PDB entry 5jdq coloured by chain, front view.
PDB entry 5jdq coloured by chain, side view.
PDB entry 5jdq coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Mechanism of extracellular ion exchange and binding-site occlusion in a sodium/calcium exchanger.
Liao J, Marinelli F, Lee C, Huang Y, Faraldo-Gómez JD, Jiang Y
Nat Struct Mol Biol 23 590-599 (2016)
PMID: 27183196

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176431 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized membrane protein MJ0091 Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 32.22 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57556 (Residues: 1-302; Coverage: 100%)
Gene name: MJ0091
Sequence domains: Sodium/calcium exchanger protein
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand NA 3 x NA
Ligand SR 1 x SR
Ligand CL 2 x CL
Ligand OLC 2 x OLC
Ligand MYS 5 x MYS
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 49.876Å b: 72.425Å c: 95.913Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.242
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structure-Functional Basis of Ion Transport in Sodium-Calcium Exchanger (NCX) Proteins.
Giladi et al. (2016)
7 more

1 mention without citation

New Insights into the Structure-Activity Relationship and Neuroprotective Profile of Benzodiazepinone Derivatives of Neurounina-1 as Modulators of the Na+/Ca2+ Exchanger Isoforms.
Magli et al. (2021)