› 5jef

X-ray diffraction
2.42Å resolution

Fragment of nitrate/nitrite sensor histidine kinase NarQ (WT) in asymmetric holo state

Released:
DOI: 10.2210/pdb5jef/pdb
PDB entry 5jef coloured by chain, front view.
PDB entry 5jef coloured by chain, side view.
PDB entry 5jef coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Mechanism of transmembrane signaling by sensor histidine kinases.
Gushchin I, Melnikov I, Polovinkin V, Ishchenko A, Yuzhakova A, Buslaev P, Bourenkov G, Grudinin S, Round E, Balandin T, Borshchevskiy V, Willbold D, Leonard G, Büldt G, Popov A, Gordeliy V
Science 356 (2017)
PMID: 28522691

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrate/nitrite sensor protein NarQ Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 26.81 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P27896 (Residues: 1-230; Coverage: 41%)
Gene names: JW2453, b2469, narQ
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NO3 1 x NO3
Ligand LFA 1 x LFA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P2
Unit cell:
a: 53.464Å b: 39.865Å c: 120.361Å
α: 90° β: 100.62° γ: 90°
R-values:
R R work R free
0.221 0.218 0.286
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
14 more

3 mentions without citation

Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.
Ishii et al. (2021)
2 more