› 5jhg

X-ray diffraction
2.5Å resolution

Crystal structure of the complex between the human RhoA and the DH/PH domain of human ARHGEF11

Released:
DOI: 10.2210/pdb5jhg/pdb
PDB entry 5jhg coloured by chain, front view.
PDB entry 5jhg coloured by chain, side view.
PDB entry 5jhg coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Wang R, Chen Q, Zhang H, Yan Z, Li J, Miao L, Wang F

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127285 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor 11 Chains: A, E
Molecule details ›
Chains: A, E
Length: 369 amino acids
Theoretical weight: 42.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15085 (Residues: 714-1081; Coverage: 24%)
Gene names: ARHGEF11, KIAA0380
Sequence domains:
Structure domains:
Transforming protein RhoA Chains: B, F
Molecule details ›
Chains: B, F
Length: 181 amino acids
Theoretical weight: 20.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61586 (Residues: 1-181; Coverage: 94%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 87.44Å b: 118.589Å c: 88.003Å
α: 90° β: 113° γ: 90°
R-values:
R R work R free
0.224 0.221 0.27
Expression system: Escherichia coli BL21(DE3)

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