› 5jif

X-ray diffraction
2Å resolution

Crystal structure of mouse hepatitis virus strain DVIM Hemagglutinin-Esterase

Released:
DOI: 10.2210/pdb5jif/pdb
PDB entry 5jif coloured by chain, front view.
PDB entry 5jif coloured by chain, side view.
PDB entry 5jif coloured by chain, top view.
Source organism: Murine coronavirus strain DVIM
Primary publication:
Coronavirus receptor switch explained from the stereochemistry of protein-carbohydrate interactions and a single mutation.
Bakkers MJ, Zeng Q, Feitsma LJ, Hulswit RJ, Li Z, Westerbeke A, van Kuppeveld FJ, Boons GJ, Langereis MA, Huizinga EG, de Groot RJ
Proc Natl Acad Sci U S A 113 E3111-9 (2016)
PMID: 27185912

Function and Biology Details

Reaction catalysed: 
N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131651 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Hemagglutinin-esterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 381 amino acids
Theoretical weight: 43.02 KDa
Source organism: Murine coronavirus strain DVIM
Expression system: Homo sapiens
UniProt:
  • Canonical: O92367 (Residues: 24-395; Coverage: 91%)
Gene names: 2b, HE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NA 2 x NA
Ligand GOL 2 x GOL
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 88.523Å b: 88.819Å c: 122.163Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.226
Expression system: Homo sapiens

Quick links

Citations

9 review citations

Biological roles of glycans.
Varki A. (2017)
8 more

2 mentions without citation

Virus recognition of glycan receptors.
Thompson et al. (2019)
1 more