5jiw

X-ray diffraction
1.73Å resolution

Crystal structure of Thermus aquaticus amylomaltase (GH77) in complex with a 34-meric cycloamylose

Released:

Function and Biology Details

Reaction catalysed:
Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-131491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
4-alpha-glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 57.23 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O87172 (Residues: 1-500; Coverage: 100%)
Gene name: malQ
Sequence domains: 4-alpha-glucanotransferase
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: I422
Unit cell:
a: 157.6Å b: 157.6Å c: 112.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.188
Expression system: Escherichia coli BL21