› 5jix

X-ray diffraction
1.47Å resolution

PKG II's Carboxyl Terminal Cyclic Nucleotide Binding Domain (CNB-B) in a complex with 8-Br-cGMP

Released:
DOI: 10.2210/pdb5jix/pdb
PDB entry 5jix coloured by chain, front view.
PDB entry 5jix coloured by chain, side view.
PDB entry 5jix coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis of Analog Specificity in PKG I and II.
Campbell JC, Henning P, Franz E, Sankaran B, Herberg FW, Kim C
ACS Chem Biol 12 2388-2398 (2017)
PMID: 28793191

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent protein kinase 2 Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13237 (Residues: 269-418; Coverage: 20%)
Gene names: PRKG2, PRKGR2
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

5 bound ligands:
Ligand CA 4 x CA
Ligand EDO 5 x EDO
Ligand PE5 1 x PE5
Ligand 6J7 1 x 6J7
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 41.62Å b: 52.09Å c: 68.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.188
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

New cGMP analogues restrain proliferation and migration of melanoma cells.
Vighi et al. (2018)
5 more

2 mentions without citation

Cyclic nucleotide selectivity of protein kinase G isozymes.
Kim et al. (2021)
1 more